FastQCFastQC Report
Thu 2 Jun 2016
DRR015215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997094
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224950.37509833929566555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190180.31712025857857157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120500.20093065074517757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.050.4236761
GTATCAA230150.038.2007181
ATCAACG284000.030.6452353
TACCTGG53400.030.3616712
TCAACGC287750.030.2933434
TATCAAC294500.029.58332
CAACGCA298350.029.2972535
AACGCAG310850.028.1920956
GTACCTG60850.028.0421831
GTGGTAT63550.026.4200151
ACAACGC10700.026.3893383
TAACGCA8200.026.1038514
ACGCAGA346150.025.106787
CGCAGAG347900.024.8235428
TATAACG7550.024.7316932
TGGTATC63750.024.7179742
ATAACGC7850.023.7867323
GCAGAGT368150.023.1246159
GTACATG172550.022.1844061
TACAACG12700.021.8747482
Produced by FastQC (version 0.11.5)