FastQCFastQC Report
Thu 2 Jun 2016
DRR015214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184450
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167290.39978969757076793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137310.32814348361194423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90060.2152254179163331No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47130.11263128965574927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.055.21411
TACCTGG46000.048.593982
TATAACG4450.046.0374532
ACAACGC9150.040.796753
GTACCTG55700.039.9345551
GTATCAA157900.038.761251
GTACCGG5300.037.056781
ACCTGGG61300.035.7204933
ATCAACG194500.031.363033
GGTACTT18450.031.1924341
TCAACGC196450.031.0969434
TAACGCA7350.030.9670524
CAACGCA205700.029.7196275
TATCAAC208650.029.2814732
GTGGTAT34950.028.881531
AACGCAG218300.028.0496836
TGGTATC35100.027.6268442
GTACAAC15550.027.3166621
GTATAAC10550.027.27491
GTACACG3600.026.6435381
Produced by FastQC (version 0.11.5)