FastQCFastQC Report
Thu 2 Jun 2016
DRR015214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184450
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196680.4700259293336042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165260.394938402896438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109570.26185042239720874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.13131952825341445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47060.1124640036324965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.054.5736471
TACCTGG48200.048.1871032
TATAACG3750.047.3631942
GTACCTG58950.040.0902631
ACCTGGG57350.039.3886873
GTATCAA170150.038.558481
ACAACGC7500.035.827293
GTACCGG5050.035.2343441
GTGGTAT38400.031.6040951
ATCAACG214500.030.1710223
TCAACGC217400.029.8104574
TGGTATC39000.029.6603812
TATCAAC223450.029.0226672
CAACGCA225250.028.9693535
AACGCAG236450.027.6913916
ATAACGC8250.027.601923
TAACGCA7150.027.3895974
GTACATG152300.025.5230541
TACATGG147850.024.6420212
ACGCAGA265050.024.4973727
Produced by FastQC (version 0.11.5)