FastQCFastQC Report
Thu 2 Jun 2016
DRR015213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5788985
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.25771357155010766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124260.21464902742017816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80890.13973088546610501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95250.052.7468381
TACCTGG58700.040.4132842
GTATCAA169950.038.8880461
TATAACG6950.038.653762
GTACCTG64550.037.5014761
TAACGCA8150.032.4000784
ATAACGC8500.032.1388663
GTCTCGC17450.030.885551
ATCAACG212550.030.8246333
TCAACGC213750.030.6712884
TATCAAC220100.029.748382
CAACGCA223400.029.6699145
TCTGTCG18200.028.4934238
AACGCAG235600.028.3064166
GTGGTAT40750.027.9087581
ACGCAGA258500.025.5165777
TGGTATC42600.025.4385222
CGCAGAG258450.025.274888
CTGTCGC22800.024.3408749
ACCTGGG96900.023.822233
Produced by FastQC (version 0.11.5)