FastQCFastQC Report
Thu 2 Jun 2016
DRR015213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5788985
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183360.316739462962851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153880.26581516448911163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103560.17889146370218614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.053.856521
TATAACG7000.046.1986122
ACAACGC9600.041.2781683
GTATCAA186400.040.827771
TACCTGG60400.040.7216572
ATAACGC8000.038.7160723
GTACCTG65800.037.997951
ATCAACG235750.032.1108443
TCAACGC239350.031.6091164
TAACGCA9450.031.3298034
TATCAAC242950.031.1214492
CAACGCA249400.030.4908395
AACGCAG262550.029.2736596
GTGGTAT43650.029.0580231
TGGTATC43200.027.6239762
GTCTCGC19600.027.4682771
TCTGTCG20500.026.8590648
GTACCGG5950.026.83841
ACCTGGG91700.026.3256383
TACAACG16350.026.1865792
Produced by FastQC (version 0.11.5)