FastQCFastQC Report
Thu 2 Jun 2016
DRR015212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5840668
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402330.6888424406249422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335280.5740439278520881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219410.3756590855703491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147920.25325870260045596No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.14138793713321832No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73050.1250713103364204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63940.10947377936907216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214650.046.5559351
TACCTGG65550.044.11132
GTATCAA365650.036.635751
ACCTGGG81200.035.5504263
GTACCTG81700.035.4426231
TATAACG7500.035.2140162
GGTACTT31350.031.3227251
TAACGCA10950.028.6905674
ATCAACG468950.028.59373
TACAACG14500.028.5773682
TCAACGC476100.028.1733654
TATCAAC476200.028.1607862
ATAACGC10900.027.9872283
CAACGCA495450.027.11625
AACGCAG514650.026.2696136
ACAACGC17650.025.0229553
GTACATG356900.024.9927671
TACATGG356350.024.2914922
GTACACG7200.024.1051431
ACGCAGA568800.023.4566297
Produced by FastQC (version 0.11.5)