FastQCFastQC Report
Thu 2 Jun 2016
DRR015212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5840668
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500700.8572649566796128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405450.6941842953579966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280290.4798937381820025No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145430.24899549161157591No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122760.2101814381505677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105500.18063002382604182No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90160.15436590472185716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79600.13628578100998037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8550.049.543762
GGTATCA230100.047.42011
TACCTGG64500.046.0426182
ACAACGC14250.039.317733
GTATCAA405900.038.5970651
ACCTGGG75700.038.089573
TACAACG14300.036.948272
GTACCTG82750.036.5632741
ATAACGC12700.033.3562663
TAACGCA12550.031.9407124
ATCAACG516700.030.1763463
TCAACGC523700.029.7735044
TATCAAC527950.029.6437032
CAACGCA541300.028.881665
AACGCAG562200.027.911066
GTATAAC18950.026.730881
GTACAAC24050.026.375411
GTACATG361500.025.1466771
ACGCAGA631600.024.6640667
CGCAGAG631150.024.5518658
Produced by FastQC (version 0.11.5)