FastQCFastQC Report
Thu 2 Jun 2016
DRR015211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6754345
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283560.41981865006895563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243480.3604790693990313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151790.22472941491736062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148950.054.820471
GTATCAA269750.039.9884151
GTGGTAT59100.031.759241
ATCAACG339400.031.497723
TCAACGC342900.031.1610974
TATCAAC349400.030.716252
TGGTATC60600.030.2054272
CAACGCA356800.029.9693265
AACGCAG374850.028.6651746
TATAACG7850.028.4222162
TACCTGG60450.028.397272
ACGCAGA420100.025.2530927
GTACATG281700.025.095731
CGCAGAG424600.024.8886558
TACATGG277400.024.3918042
GCAGAGT446600.023.0718149
GTACCTG77650.022.584241
ACATGGG290750.022.2519073
TAACGCA10950.020.7884924
ACCTGGG80200.020.7210333
Produced by FastQC (version 0.11.5)