FastQCFastQC Report
Thu 2 Jun 2016
DRR015211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6754345
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345430.511418945878542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297500.4404572167989642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187420.2774806439410483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.1170061641802425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163050.056.828921
GTATCAA300750.040.6394041
GTGGTAT60400.034.5191081
TATAACG7500.033.3993032
ATCAACG378750.031.987323
TCAACGC381000.031.7745114
TACCTGG61350.031.2538492
TATCAAC390200.031.11782
TGGTATC64900.030.8074552
CAACGCA394600.030.7229125
AACGCAG411500.029.5140366
ACAACGC13750.028.155623
ACGCAGA465150.025.884917
CGCAGAG470950.025.4888298
GTACATG286650.025.0037141
GTACCTG80200.024.745441
TACATGG281000.024.247182
GCAGAGT501100.023.5926919
ACCTGGG79400.023.4612793
ATAACGC11500.022.9709533
Produced by FastQC (version 0.11.5)