FastQCFastQC Report
Thu 2 Jun 2016
DRR015210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8356654
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233160.2790111927572926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201030.2405627898438777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120280.14393320580222657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149200.051.63851
GTATCAA269450.039.2712941
ATCAACG332650.031.5625173
TCAACGC338850.030.8891054
TATCAAC350100.030.05692
CAACGCA350500.029.8734255
AACGCAG368650.028.5781276
GTGGTAT67100.028.0415521
TGGTATC68200.026.1720662
ACGCAGA413600.025.0986147
CGCAGAG415050.024.8466788
TACCTGG65850.023.9252592
GTACCTG71850.023.0096041
TATAACG7800.022.7670272
GCAGAGT443550.022.7373779
GTACATG240050.022.677851
TACATGG234250.021.9263252
ACATGGG245700.020.2171273
GAGTACT306850.020.00974812-13
CAGAGTA435400.019.68112410-11
Produced by FastQC (version 0.11.5)