FastQCFastQC Report
Thu 2 Jun 2016
DRR015210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8356654
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283620.33939421208536336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249980.2991388658666495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150640.18026353609949627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162400.053.7244761
GTATCAA297800.039.778681
ATCAACG369550.031.701053
TCAACGC374400.031.2911424
TATCAAC386100.030.482672
CAACGCA387200.030.3465465
AACGCAG404350.029.1368226
GTGGTAT72000.028.7735671
TGGTATC72300.027.4668272
TACCTGG66900.026.14362
ACGCAGA455900.025.6226397
CGCAGAG455800.025.438578
GTACCTG73750.024.687731
GTACATG255300.024.5408741
ACAACGC12800.023.4860443
TACATGG249950.023.3976482
GCAGAGT490100.023.3429629
ACATGGG254950.021.5996743
TATAACG8300.021.4016282
GAGTACT356150.020.14175812-13
Produced by FastQC (version 0.11.5)