FastQCFastQC Report
Thu 2 Jun 2016
DRR015209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156756
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184420.2995408621033544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153090.24865367411019698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95950.15584505866401072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112150.049.0772251
GTATCAA205600.039.0782361
TACCTGG58650.033.300012
ATCAACG251500.031.7657343
TCAACGC252900.031.553634
GTACCTG64100.031.068531
TATCAAC256900.031.029672
CAACGCA264800.030.2034055
AACGCAG278200.029.1069376
ACGCAGA307950.026.0436737
CGCAGAG307700.025.8282858
GTGGTAT55000.025.7449021
TATAACG6300.025.2919672
GTCTCGC17000.024.1816441
GCAGAGT325500.023.7170689
ACAACGC13500.023.6039163
TGGTATC57500.023.3565832
GTACATG161650.021.9269521
TACAACG14750.021.6053432
ACCTGGG89900.020.8620643
Produced by FastQC (version 0.11.5)