FastQCFastQC Report
Thu 2 Jun 2016
DRR015209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156756
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221780.3602221689474132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190340.309156315436246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123120.19997544161243358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127950.051.2102741
GTATCAA227500.038.509051
TATAACG5750.034.8506662
ACAACGC10700.033.6272053
TACCTGG63350.032.4232252
ATAACGC7150.031.2131463
GTACCTG67800.030.823271
ATCAACG287300.030.1841413
TCAACGC289000.030.0228334
TATCAAC292450.029.7445952
CAACGCA300750.028.8831985
AACGCAG316150.027.5907426
TACAACG14500.025.4415342
ACGCAGA351250.024.6781777
CGCAGAG351550.024.553578
GTGGTAT60600.023.944041
TGGTATC61000.023.070392
ACCTGGG84950.023.0543333
GTACATG166800.023.0060921
GCAGAGT373650.022.5415069
Produced by FastQC (version 0.11.5)