FastQCFastQC Report
Thu 2 Jun 2016
DRR015208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14864288
Sequences flagged as poor quality0
Sequence length97
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282730.19020756325496382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231130.15549348882368264No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA159290.10716288597206944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271650.044.3436851
GTATCAA452650.039.6103361
ATCAACG601150.029.613613
TCAACGC602400.029.5746354
TATCAAC622550.028.7658942
CAACGCA673000.026.4571975
AACGCAG691500.025.7640616
ACGCAGA724650.024.2779037
CGCAGAG747050.023.4461738
GTACATG655000.022.9727881
GCAGAGT775350.022.0153689
TACATGG664650.021.4632532
ACATGGG703150.019.6909143
AGAGTAC706350.019.08714110-11
GAGTACA495050.018.8609351
GTACTTT378200.018.7711214-15
CAGAGTA749700.018.60536210-11
GAGTACT388750.018.39280312-13
GTGGTAT139450.018.344291
AGTACTT389350.018.06656512-13
Produced by FastQC (version 0.11.5)