FastQCFastQC Report
Thu 2 Jun 2016
DRR015208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14864288
Sequences flagged as poor quality0
Sequence length97
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360550.24256123132167512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294460.19809896040765626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182800.12297931794647682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA297600.046.2073861
GTATCAA511200.040.8499151
ATCAACG681350.030.5165253
TCAACGC686400.030.2794534
TATCAAC702300.029.6705572
CAACGCA731050.028.4083755
AACGCAG755600.027.507796
ACGCAGA831150.024.7336567
CGCAGAG855750.023.9163048
GTACATG697200.023.0283581
GCAGAGT895500.022.4846069
TACATGG703700.021.6503962
ACATGGG737200.020.0305213
AGAGTAC817950.019.59568210-11
GTGGTAT149750.019.3162821
CAGAGTA868750.018.82170510-11
GAGTACT483700.018.67224112-13
GTACTTT479750.018.57867214-15
AGTACTT481200.018.48558212-13
GAGTACA522400.018.2446841
Produced by FastQC (version 0.11.5)