FastQCFastQC Report
Thu 2 Jun 2016
DRR015207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741739
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169870.219420985388425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147520.1905515027050124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.1087998445827223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175600.035.52961
GTATCAA275650.031.9623221
ATCAACG326000.026.5211243
TCAACGC328600.026.3251324
TATCAAC334500.025.9984282
CAACGCA339600.025.4706215
AACGCAG349750.024.8447276
GTACATG169500.022.5538061
CGCAGAG382500.022.2881628
ACGCAGA384650.022.2827177
TACATGG171950.021.077582
GAGTACT253050.020.6883812-13
AGAGTAC326300.020.47746710-11
GCAGAGT412950.020.1379329
TCTGTCG21200.019.741868
ACATGGG179450.019.3328573
AGTACTT274150.018.57348312-13
GTACTTT284950.018.38656214-15
CTGTCGC23800.017.9674749
CAGAGTA412350.017.03701210-11
Produced by FastQC (version 0.11.5)