FastQCFastQC Report
Thu 2 Jun 2016
DRR015207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741739
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200870.2594636683050152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176590.2281012056851826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105220.13591261601560062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199350.035.0182341
GTATCAA310500.031.5345881
ATCAACG364100.026.4570373
TCAACGC368100.026.2444554
TATCAAC373850.025.8272842
CAACGCA376700.025.7318155
AACGCAG387500.025.0600016
ACGCAGA428350.022.5001567
CGCAGAG427500.022.459748
GTACATG172600.022.390391
GAGTACT288350.021.34185812-13
TACATGG173600.021.0671632
GCAGAGT458100.020.7016169
AGAGTAC364250.020.57599410-11
ACATGGG176450.019.9788633
AGTACTT311100.019.18152612-13
GTACTTT329650.018.52877214-15
TCTGTCG19550.017.4571328
ACAACGC10250.017.3297463
CAGAGTA461300.017.26320510-11
Produced by FastQC (version 0.11.5)