FastQCFastQC Report
Thu 2 Jun 2016
DRR015206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527465
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288320.5216134340063664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243890.4412330064505157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161010.2912908539448011No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102120.18475015219454125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60910.11019517988806804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.10067906354902292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141400.051.7989351
GTATCAA253600.038.95321
TACCTGG52450.033.3416372
TATAACG5700.031.9584982
ATCAACG325500.030.233013
TATCAAC329250.029.9594542
TCAACGC328350.029.9412274
GTACCTG61750.028.9318711
CAACGCA344200.028.6529985
AACGCAG356750.027.7520286
TACAACG13450.026.7489072
GGTACTT29450.025.2894291
ACGCAGA396100.024.8108047
CGCAGAG398050.024.6325558
ATAACGC7800.024.5206683
ACCTGGG69200.024.4801713
GTACATG257350.024.4126681
GTGGTAT61850.024.0832251
ACAACGC16100.023.7591573
TACATGG258150.023.3746412
Produced by FastQC (version 0.11.5)