FastQCFastQC Report
Thu 2 Jun 2016
DRR015206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527465
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352950.638538642940299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296430.5362856209853885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204460.3698983168595369No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120020.21713389410878223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83710.15144374500788335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78830.1426151047541685No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71720.12975206536812084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156400.053.1132771
GTATCAA278950.039.3131681
TACCTGG56800.032.5597882
ATCAACG365600.029.890953
TCAACGC369750.029.5559944
TATCAAC368850.029.5403212
GTACCTG65550.029.1589051
TAACGCA7750.028.8016834
TACAACG14400.028.7860952
ACAACGC14600.028.7045423
CAACGCA385050.028.5426865
TATAACG6950.028.1830162
AACGCAG398000.027.7834786
GTGGTAT70450.027.7783361
ATAACGC8000.026.7623063
TGGTATC70500.026.5556282
ACCTGGG71450.025.1831783
ACGCAGA446700.024.560927
CGCAGAG449000.024.3236268
GTACATG255250.024.0552121
Produced by FastQC (version 0.11.5)