FastQCFastQC Report
Thu 2 Jun 2016
DRR015205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632007
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230160.34704426578560604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198330.29904974467005235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120980.18241838405779728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146050.046.657421
GTATCAA251350.036.5233541
ATCAACG304400.029.6457143
TCAACGC308400.029.3328044
TATCAAC316600.028.7778782
CAACGCA320200.028.2507675
AACGCAG331950.027.383856
GTACATG215150.025.0704421
ACGCAGA371650.024.2872967
CGCAGAG375750.023.8889188
TACATGG219400.023.5341782
TACCTGG49100.023.0908782
GTGGTAT73250.022.758241
GCAGAGT397800.021.992929
ACATGGG229850.021.789693
TGGTATC74100.021.5066322
GAGTACT262300.020.30696512-13
GTACCGG6800.019.4779191
AGAGTAC358100.019.44144610-11
GTACTTT274300.019.32569914-15
Produced by FastQC (version 0.11.5)