FastQCFastQC Report
Thu 2 Jun 2016
DRR015205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632007
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280970.42365757454719216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247780.3736123921461482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155580.23458961970335676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.10717720895047306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170000.045.8458941
GTATCAA294400.036.5948261
ATCAACG360250.029.6760313
TCAACGC364900.029.2983074
TATCAAC369200.029.0672362
CAACGCA377100.028.3974025
AACGCAG390750.027.5183416
ACGCAGA439050.024.2837547
CGCAGAG443400.023.9736798
GTACATG218900.023.305451
GTGGTAT88850.022.8540951
GCAGAGT462200.022.4477219
TACATGG223200.022.142452
ACAACGC11750.022.097023
TGGTATC89900.021.483072
ACATGGG226850.021.083723
TACCTGG50850.020.8715692
GAGTACT312750.020.7391712-13
AGAGTAC408150.020.35869610-11
CAGAGTA457250.019.17274310-11
Produced by FastQC (version 0.11.5)