FastQCFastQC Report
Thu 2 Jun 2016
DRR015204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577096
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217750.33107316663767716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190440.28955028176569114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113290.17224927232322593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126250.052.1299441
GTATCAA233650.039.01661
ATCAACG294000.030.754933
TCAACGC296800.030.464794
TATCAAC304250.029.8702752
CAACGCA308900.029.312985
AACGCAG322900.028.2762326
TGGTATC55900.027.0417982
GTGGTAT57300.027.0208971
ACGCAGA357700.025.206957
CGCAGAG359000.025.0144848
GTACATG191400.023.6475641
GCAGAGT384200.022.698849
TACATGG186950.022.6499252
TACCTGG48500.021.967642
ACATGGG197450.020.660163
AGAGTAC355050.020.29317510-11
GAGTACT277050.019.93104212-13
GTACACG7350.019.8846231
CAGAGTA382400.019.50194210-11
Produced by FastQC (version 0.11.5)