FastQCFastQC Report
Thu 2 Jun 2016
DRR015204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577096
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255130.38790676006553654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224160.3408191092238885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136560.2076296286385359No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.10387563143369048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139100.052.146461
GTATCAA253200.040.0165561
ATCAACG320500.031.2938633
TCAACGC324400.030.8481454
GTGGTAT61050.030.5627541
TATCAAC333350.030.2098312
CAACGCA336850.029.8565045
TGGTATC62550.029.418062
AACGCAG352850.028.5658426
ACGCAGA390800.025.535887
CGCAGAG390500.025.4622698
TACCTGG52400.023.7296492
GCAGAGT418250.023.5124979
ACAACGC12400.023.5086783
TATAACG5300.023.20322
GTACATG192600.022.2415031
TACATGG190400.021.0511442
GTACCTG61250.020.8548361
AGAGTAC389250.020.76319910-11
GAGTACT308350.020.40939312-13
Produced by FastQC (version 0.11.5)