FastQCFastQC Report
Thu 2 Jun 2016
DRR015203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973458
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292440.20928248397783852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231890.16595033240877097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149840.10723186773095106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226550.053.3033181
GTATCAA426000.041.7808881
ATCAACG546950.032.323263
TCAACGC553650.031.914744
TATCAAC585950.030.2512022
CAACGCA611500.028.9452255
AACGCAG632900.028.0038766
ACGCAGA675000.025.9199167
CGCAGAG695600.025.0474878
GTGGTAT89050.024.9263781
GTACATG644100.024.7899111
TACATGG627200.024.0800912
GCAGAGT726450.023.4139529
ACATGGG673600.021.7780443
TGGTATC101100.020.9871522
CAGAGTA720600.019.46069510-11
AGAGTAC698150.019.2134410-11
GTACTTT374750.019.07256314-15
CATGGGG450000.018.8892524
AGTACTT388500.018.33439412-13
Produced by FastQC (version 0.11.5)