FastQCFastQC Report
Thu 2 Jun 2016
DRR015203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973458
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360120.2577171663592505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285370.20422289171370464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192600.1378327397556138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248900.053.312711
GTATCAA473500.042.383471
ATCAACG606800.032.764183
TCAACGC614550.032.3372154
TATCAAC640700.031.193322
CAACGCA652600.030.4523125
AACGCAG676400.029.412876
GTGGTAT97400.027.8752821
ACGCAGA751600.026.2217857
CGCAGAG776800.025.2129638
GTACATG669000.024.79361
TACATGG648850.023.985592
TGGTATC110200.023.8062292
GCAGAGT810800.023.7017619
ACATGGG686900.022.0607323
CATGGGG445550.019.587264
AGAGTAC776600.019.55656210-11
CAGAGTA805550.019.51467510-11
GTACTTT451200.019.41676914-15
AGTACTT457050.019.0149312-13
Produced by FastQC (version 0.11.5)