FastQCFastQC Report
Thu 2 Jun 2016
DRR015202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134447
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280280.251723323125073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252500.22677372302369395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140370.12606822772608284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224650.058.685511
GTATCAA436250.042.40891
GTGGTAT80700.032.887721
ATCAACG561200.032.6330343
TATCAAC567300.032.4105342
TCAACGC566200.032.3435484
CAACGCA601050.030.4573545
TGGTATC87050.028.9188772
AACGCAG635450.028.8934786
GTACATG556100.026.572621
TACATGG552150.026.0198652
ACGCAGA711150.025.7648037
CGCAGAG713700.025.6523558
ACATGGG565100.024.3763523
GCAGAGT758000.023.9238789
CATGGGT174200.022.0547334
CATGGGG285250.020.5700244
CAGAGTA735950.020.48991610-11
GAGTACT439050.020.47603412-13
AGAGTAC699250.019.96175210-11
Produced by FastQC (version 0.11.5)