FastQCFastQC Report
Thu 2 Jun 2016
DRR015202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134447
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313100.28119941654938047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292140.2623749522540275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157900.14181216184333179No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243250.058.5021671
GTATCAA462400.041.7169841
GTGGTAT85450.035.652411
ATCAACG601950.031.7723733
TATCAAC605200.031.7104072
TCAACGC605850.031.672974
TGGTATC94600.031.0214982
CAACGCA636100.030.1739035
AACGCAG669200.028.7470976
GTACATG558400.025.4683821
ACGCAGA765800.025.1205257
CGCAGAG768350.025.060958
TACATGG558150.024.8216442
GCAGAGT814150.023.3328789
ACATGGG574750.023.1285613
CATGGGT175400.020.4123274
GAGTACT494600.020.39392712-13
CAGAGTA789250.020.33397310-11
AGAGTAC756250.019.873710-11
AGTACTT490800.019.83339512-13
Produced by FastQC (version 0.11.5)