FastQCFastQC Report
Thu 2 Jun 2016
DRR015201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665505
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307960.2887439460203713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296330.2778396334725829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155640.14592839251399722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA245550.058.7624741
GTATCAA457500.041.172971
GTGGTAT84350.035.5728071
TGGTATC90000.032.6816332
ATCAACG583400.032.052043
TCAACGC587950.031.7862724
TATCAAC593800.031.5608652
CAACGCA620200.030.1366445
AACGCAG642050.029.1287426
ACGCAGA724750.025.7464687
GTACATG526200.025.6215481
CGCAGAG734400.025.3935078
TACATGG524550.024.9640882
GCAGAGT782250.023.5940349
CATGGGT188900.023.4984654
ACATGGG540150.023.1379763
GAGTACT484250.020.92229812-13
CAGAGTA760900.020.80090110-11
GTACTTT505850.019.61997214-15
CATGGGG256550.019.325144
Produced by FastQC (version 0.11.5)