FastQCFastQC Report
Thu 2 Jun 2016
DRR015201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665505
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345480.3239227772149561No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344000.3225351260910759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177200.16614309402133326No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT126590.11869105119729445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257150.060.081041
GTATCAA480700.041.2176481
GTGGTAT83950.035.1036031
TGGTATC87850.032.267252
ATCAACG630950.031.1408633
TCAACGC636950.030.9546534
TATCAAC638100.030.8755572
CAACGCA664500.029.6781235
AACGCAG686950.028.7436456
GTACATG540850.025.2646061
ACGCAGA785900.025.1070337
CGCAGAG793800.024.9144638
TACATGG532850.024.8401622
CATGGGT198100.023.9060944
GCAGAGT841750.023.3873679
ACATGGG549350.023.1211073
GAGTACT542350.020.75796512-13
CAGAGTA823600.020.59148410-11
AGAGTAC791500.019.82879310-11
GTACTTT559350.019.79152514-15
Produced by FastQC (version 0.11.5)