FastQCFastQC Report
Thu 2 Jun 2016
DRR015200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14307330
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386290.269994471365377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338130.23633340392651878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194350.13583946131109018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301000.061.3958851
GTATCAA608100.043.82181
GTGGTAT99750.035.0994341
ATCAACG791350.033.3559653
TATCAAC803050.033.0811352
TCAACGC799650.033.0264784
TGGTATC103250.032.498222
CAACGCA836450.031.5528285
AACGCAG881300.030.050836
GTACATG856300.027.2652051
TACATGG851050.026.7323062
CGCAGAG999800.026.3661488
ACGCAGA1005000.026.3041827
ACATGGG874850.024.9111483
GCAGAGT1062200.024.4169339
CATGGGT231300.023.2845844
CATGGGG401650.021.399994
CAGAGTA1030450.020.75386410-11
GAGTACT603650.020.30979712-13
CATGGGA283450.020.1891564
Produced by FastQC (version 0.11.5)