FastQCFastQC Report
Thu 2 Jun 2016
DRR015200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14307330
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431230.30140494417896285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397560.2778715525538308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219640.15351571537107203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA323200.061.6246451
GTATCAA638500.043.1844981
GTGGTAT104300.036.8120161
TGGTATC110150.033.7498782
ATCAACG846550.032.282813
TATCAAC857650.032.0185622
TCAACGC857750.031.9726644
CAACGCA889950.030.805725
AACGCAG938900.029.27376
GTACATG854100.027.0628591
TACATGG847250.026.5092982
CGCAGAG1083150.025.3999798
ACGCAGA1087900.025.2345437
ACATGGG877250.024.4836943
GCAGAGT1144600.023.7980449
CATGGGT239150.023.5133464
CATGGGG404150.021.6585224
CAGAGTA1116800.020.44159310-11
GAGTACT687100.020.28819812-13
AGAGTAC1080700.020.16250810-11
Produced by FastQC (version 0.11.5)