FastQCFastQC Report
Thu 2 Jun 2016
DRR015199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15128632
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365190.24138996837255342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313800.2074212658487562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181490.11996458106721082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA305800.060.8487131
GTATCAA609300.041.0825921
GTGGTAT99600.037.0262681
TGGTATC101600.035.3108442
ATCAACG768750.032.127213
TCAACGC782850.031.5709864
TATCAAC795400.031.2294542
CAACGCA853350.028.9533755
AACGCAG895950.027.607686
GTACATG831800.027.4496151
TACATGG825850.027.1672172
CGCAGAG975250.025.2428478
ACATGGG852650.025.158973
ACGCAGA986500.025.011487
CATGGGT176900.023.8560644
GCAGAGT1037000.023.4227479
CATGGGA328200.022.3878214
CATGGGG356000.021.4324054
GCTCTAG43100.020.704421
CAGAGTA1027100.020.11126710-11
Produced by FastQC (version 0.11.5)