FastQCFastQC Report
Thu 2 Jun 2016
DRR015199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15128632
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347820.22990842793981636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316520.20921918121876454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175550.11603825117829555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303000.060.2598461
GTATCAA598750.040.885331
GTGGTAT100850.038.2057761
TGGTATC102500.036.6229862
ATCAACG764600.031.7018553
TCAACGC780350.031.2369084
TATCAAC795700.030.5591182
CAACGCA836000.029.146875
AACGCAG879400.027.7572176
GTACATG852800.026.9645231
TACATGG846050.026.513962
CGCAGAG981550.024.8402218
ACGCAGA989600.024.6105757
ACATGGG873600.024.5488823
CATGGGT179500.023.7228134
GCAGAGT1046400.022.9487959
CATGGGA330050.021.9992524
CATGGGG370100.020.9584944
AGTACAT625000.019.6492772
CAGAGTA1040000.019.590310-11
Produced by FastQC (version 0.11.5)