FastQCFastQC Report
Thu 2 Jun 2016
DRR015198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874591
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA289200.049.0587961
GTATCAA513950.040.265281
TCAACGC641950.031.7427924
ATCAACG642800.031.7165833
TATCAAC657250.031.2217312
CAACGCA681300.029.8847835
AACGCAG719850.028.3699676
GTACATG1001050.027.895551
TACATGG995550.027.2527482
GTGGTAT120450.027.1415941
ACATGGG1046450.024.7207053
ACGCAGA838050.024.312347
CGCAGAG879850.023.0775248
TGGTATC137100.022.4164242
CATGGGG646450.021.4582234
GAGTACA752550.021.382031
GCAGAGT943800.021.0829459
AGTACAT723550.020.7719362
CATGGGA288700.019.8534154
CATGGGT205850.019.130294
Produced by FastQC (version 0.11.5)