FastQCFastQC Report
Thu 2 Jun 2016
DRR015198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874591
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233070.11165248698764925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296500.051.245131
GTATCAA534000.040.6503261
ATCAACG677550.031.5375273
TCAACGC680850.031.4447294
TATCAAC691050.031.1094072
CAACGCA712650.030.0098235
AACGCAG751850.028.4774466
GTGGTAT119700.028.081061
GTACATG986700.026.9851591
TACATGG986850.026.2199272
ACGCAGA880950.024.288117
ACATGGG1036650.023.8032323
CGCAGAG925650.023.1692818
TGGTATC138250.023.0373382
GCAGAGT990800.021.3247859
GAGTACA758250.021.1016791
CATGGGG645350.020.4644034
AGTACAT723200.020.4593352
CATGGGT204700.019.4463274
CATGGGA287700.018.943684
Produced by FastQC (version 0.11.5)