FastQCFastQC Report
Thu 2 Jun 2016
DRR015197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12150230
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253810.20889316498535418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225430.18553558245399468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130100.10707616234425191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223300.058.9874841
GTATCAA435800.041.5288661
GTGGTAT78850.038.1103021
TGGTATC84350.034.8679242
ATCAACG560800.032.0019573
TCAACGC569550.031.5081064
TATCAAC583100.030.974042
CAACGCA630000.028.5095025
AACGCAG665600.027.1002676
GTACATG590250.025.6327081
CGCAGAG709800.025.34398
ACGCAGA714750.025.2216937
TACATGG580750.025.1335012
GCAGAGT749000.023.4753389
ACATGGG602300.023.1376083
CATGGGT103250.020.7667434
CATGGGG299300.020.1381174
CAGAGTA731200.019.91290710-11
CATGGGA207700.019.682374
GTACTTT410850.019.47881514-15
Produced by FastQC (version 0.11.5)