FastQCFastQC Report
Thu 2 Jun 2016
DRR015197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12150230
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278940.22957590103232614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262480.21602883237601261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144040.1185491961880557No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT133840.11015429337551635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243600.060.2909741
GTATCAA467750.041.0474931
GTGGTAT80750.038.8576551
TGGTATC85750.035.6570242
ATCAACG600850.031.5960833
TCAACGC609150.031.2924024
TATCAAC621000.030.7508582
CAACGCA662450.028.7746525
AACGCAG699300.027.3074746
CGCAGAG763800.024.9710718
GTACATG604650.024.7869831
ACGCAGA770200.024.7576667
TACATGG594850.024.3160482
GCAGAGT804750.023.3445019
ACATGGG619050.022.2011743
CATGGGT107850.020.6692684
CAGAGTA787350.020.20839710-11
GTACTTT462400.019.63647314-15
GAGTACT483700.019.38557612-13
CATGGGG311450.019.3104444
Produced by FastQC (version 0.11.5)