FastQCFastQC Report
Thu 2 Jun 2016
DRR015196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11442953
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248360.21704187721473647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233880.2043878009461369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125930.1100502641232556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA235400.056.8061831
GTATCAA426000.039.833451
GTGGTAT85450.033.0885471
ATCAACG542400.030.9808963
TCAACGC547550.030.6878934
TATCAAC560600.030.1468282
CAACGCA583000.028.8251465
TGGTATC95300.028.473452
AACGCAG609700.027.6293956
GTACATG539350.025.4769081
ACGCAGA672350.025.025287
TACATGG536650.024.9172172
CGCAGAG679100.024.713438
ACATGGG546000.023.3722253
GCAGAGT720200.023.036789
CATGGGT161950.021.2795164
CAGAGTA690900.020.63393810-11
GAGTACT410300.020.61933312-13
GTACTTT417200.019.81496414-15
GAGTACA419350.019.6625541
Produced by FastQC (version 0.11.5)