FastQCFastQC Report
Thu 2 Jun 2016
DRR015196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11442953
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279220.24401044031204183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274220.23964093883807788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143280.12521243423791042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246250.057.7984161
GTATCAA447200.040.0455131
GTGGTAT86200.033.287061
ATCAACG576850.030.7037033
TCAACGC585300.030.3847984
TATCAAC593400.030.08042
TGGTATC95250.029.521362
CAACGCA616900.028.8284995
AACGCAG647400.027.5230776
GTACATG549950.024.9113221
ACGCAGA726300.024.4828457
CGCAGAG732100.024.3448078
TACATGG546450.024.2800712
ACATGGG561100.022.5734633
GCAGAGT783050.022.5517489
CATGGGT162700.021.3915924
CAGAGTA753650.020.41233810-11
GAGTACT465700.020.3493412-13
GTACTTT473650.019.73688314-15
AGAGTAC724850.019.20239410-11
Produced by FastQC (version 0.11.5)