FastQCFastQC Report
Thu 2 Jun 2016
DRR015195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7982429
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188670.23635662778835864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164230.20573938083257615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95220.11928699898238995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166150.056.781341
GTATCAA304250.040.630811
GTGGTAT57100.033.7303241
ATCAACG400750.030.5388683
TATCAAC407300.030.1489282
TCAACGC405850.030.1424
TGGTATC65200.028.4916732
CAACGCA435850.028.073345
AACGCAG457800.026.7474166
GTACATG394100.025.5907821
TACATGG390650.025.4198632
ACGCAGA504300.024.2612447
CGCAGAG504050.024.2179038
ACATGGG405650.023.3794863
GCAGAGT530850.022.7021479
CATGGGT110300.021.009334
CATGGGG212200.020.4893384
CAGAGTA505300.020.1518210-11
GAGTACT302100.019.59617412-13
AGAGTAC487050.019.44992310-11
Produced by FastQC (version 0.11.5)