FastQCFastQC Report
Thu 2 Jun 2016
DRR015195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7982429
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204290.25592460640739806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189270.23710827869562007No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT131040.1641605581458977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106400.1332927608876947No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG101770.12749252138666062RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT81190.10171089526759336RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175100.058.78411
GTATCAA327850.040.9950681
GTGGTAT57900.035.2400931
ATCAACG425000.031.4074233
TATCAAC430350.031.1485182
TCAACGC431800.031.091934
TGGTATC64650.030.1232412
CAACGCA458200.029.2909535
AACGCAG477500.028.1421596
GTACATG395500.025.5764161
TACATGG397500.025.04592
ACGCAGA536900.025.010927
CGCAGAG541450.024.8091458
ACATGGG408900.023.3648663
GCAGAGT570750.023.1613889
CATGGGT109250.022.8202424
GAGTACT335050.020.61984312-13
CAGAGTA546100.020.46881710-11
CATGGGG213950.020.456154
GTACTTT340450.019.70700514-15
Produced by FastQC (version 0.11.5)