FastQCFastQC Report
Thu 2 Jun 2016
DRR015194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8709332
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213690.24535750847481758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201710.23160214813260074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109070.12523348518577543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196250.057.516331
GTATCAA354550.041.1214261
GTGGTAT69450.033.502171
ATCAACG464500.031.1797263
TCAACGC467450.030.9813544
TATCAAC473850.030.642062
TGGTATC74600.030.577592
CAACGCA501650.028.8747885
AACGCAG528050.027.5141546
GTACATG468050.025.5462281
TACATGG467750.024.957932
ACGCAGA585550.024.7946347
CGCAGAG591000.024.541348
ACATGGG480000.023.2201233
GCAGAGT625750.022.835459
CATGGGG240550.021.4435374
GAGTACT320500.020.89806712-13
CAGAGTA594850.020.4636210-11
CATGGGA202250.020.2818764
AGTACTT326700.019.61040712-13
Produced by FastQC (version 0.11.5)