FastQCFastQC Report
Thu 2 Jun 2016
DRR015194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8709332
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228130.261937425281296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224260.25749391572166497No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT122970.14119337740253787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118770.13637096392696937No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG88110.10116734555531928RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203300.058.4231831
GTATCAA368550.041.0751881
GTGGTAT67450.037.406131
TGGTATC73950.032.3657532
ATCAACG488200.030.7540263
TCAACGC492900.030.4975224
TATCAAC498650.030.2500342
CAACGCA520450.028.8306855
AACGCAG545600.027.522616
GTACATG467050.024.3581371
ACGCAGA618200.024.2382137
CGCAGAG624700.024.0224238
TACATGG467050.023.5965732
GCAGAGT655700.022.623599
ACATGGG484250.021.7316063
GAGTACT352950.020.91491312-13
CAGAGTA629450.020.20862810-11
AGTACTT361050.019.78412612-13
AGAGTAC588750.019.75880410-11
CATGGGA207300.019.511284
Produced by FastQC (version 0.11.5)