FastQCFastQC Report
Thu 2 Jun 2016
DRR015193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17002126
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236540.13912377781460977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206840.12165537415732598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266500.053.5583721
GTATCAA493850.041.3295561
ATCAACG633250.031.8210373
TCAACGC636050.031.694434
TATCAAC639850.031.6000962
GTGGTAT103950.029.9584031
CAACGCA690200.029.2169765
AACGCAG719900.028.0591816
GTACATG777800.026.4521791
TACATGG767550.025.9452532
ACGCAGA802750.025.1438067
TGGTATC120350.025.0814842
CGCAGAG825150.024.4154748
ACATGGG801250.023.8535213
GCAGAGT881850.022.4725139
CATGGGG493350.020.4217474
CAGAGTA839900.019.26168610-11
CATGGGA229750.019.241124
GAGTACA595400.019.2392831
AGAGTAC800600.018.9913110-11
Produced by FastQC (version 0.11.5)