FastQCFastQC Report
Thu 2 Jun 2016
DRR015193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17002126
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279090.1641500598219305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251420.14787562449543074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280150.055.121931
GTATCAA522350.041.2892841
ATCAACG673100.031.6301333
TCAACGC676900.031.580264
TATCAAC682450.031.4209182
GTGGTAT108400.030.9197351
CAACGCA722900.029.5646085
AACGCAG758600.028.2720726
TGGTATC122550.026.322382
GTACATG761300.025.3927121
ACGCAGA863200.024.814317
TACATGG755700.024.7689232
CGCAGAG887050.024.1112378
ACATGGG792500.022.5192413
GCAGAGT939900.022.4411059
GAGTACA601050.019.8129121
CATGGGG490350.019.4640884
CAGAGTA898700.019.40775710-11
AGAGTAC856600.019.28616710-11
AGTACAT579050.019.1797942
Produced by FastQC (version 0.11.5)