FastQCFastQC Report
Thu 2 Jun 2016
DRR015192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14230421
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258100.1813720057895687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229840.16151314145941292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248600.055.895811
GTATCAA467950.042.032011
GTGGTAT90900.032.2565421
ATCAACG614900.031.6520233
TCAACGC619000.031.4486264
TATCAAC634550.030.9169982
CAACGCA667800.029.1261485
TGGTATC99350.028.6875292
AACGCAG690400.028.2485856
GTACATG786250.027.5234581
TACATGG775950.027.2662642
ACATGGG805300.025.1747343
ACGCAGA777400.025.061987
CGCAGAG790700.024.6272728
CATGGGT186900.023.4082364
GCAGAGT848300.022.7232469
CATGGGG399800.021.4646194
CATGGGA255500.021.0789244
GAGTACA593650.019.9482921
AGTACAT565050.019.7247372
Produced by FastQC (version 0.11.5)