FastQCFastQC Report
Thu 2 Jun 2016
DRR015192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14230421
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292240.20536286312260194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267000.18762621288576073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154620.10865455069811357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267000.057.9732551
GTATCAA498650.041.2060551
GTGGTAT92950.032.727651
ATCAACG653000.031.2674353
TCAACGC657550.031.1410164
TATCAAC674200.030.3955172
CAACGCA698350.029.3022125
TGGTATC104150.028.701592
AACGCAG731500.028.083586
GTACATG794150.026.188841
TACATGG784850.025.7682532
ACGCAGA837650.024.4917147
CGCAGAG847500.024.2446358
ACATGGG810750.023.719083
GCAGAGT905250.022.4370529
CATGGGT187900.021.7179184
CATGGGA255950.020.8850384
CATGGGG403400.020.2094574
CAGAGTA875750.019.65489410-11
GAGTACA607800.019.2857111
Produced by FastQC (version 0.11.5)