FastQCFastQC Report
Thu 2 Jun 2016
DRR015191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13371990
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338350.2530289059444406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307400.2298835102329571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167270.12508983330080264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA290250.057.3685261
GTATCAA536850.040.3486141
GTGGTAT105450.034.4232181
TGGTATC109450.031.9990652
ATCAACG684700.031.3285853
TCAACGC694050.030.9112454
TATCAAC702100.030.7148022
CAACGCA731100.029.30425
AACGCAG764500.028.1027156
GTACATG616300.025.4735131
CGCAGAG851750.025.1548798
ACGCAGA855050.025.1181147
TACATGG605950.025.0209452
GCAGAGT909950.023.2450129
ACATGGG638400.022.7642923
GAGTACT533150.020.84668412-13
CATGGGT179100.020.7485964
CATGGGG329700.020.4840774
CAGAGTA881100.020.4787110-11
GTACTTT559400.019.33538814-15
Produced by FastQC (version 0.11.5)