FastQCFastQC Report
Thu 2 Jun 2016
DRR015191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13371990
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363510.27184435525303263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341870.25566127405120703No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT205310.1535373568182447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188610.1410485649480743No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG157050.11744699180899776TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA309000.058.7231831
GTATCAA582150.040.915661
GTGGTAT112550.036.2495231
TGGTATC112100.035.718652
ATCAACG747500.031.451753
TATCAAC756400.031.2381112
TCAACGC756150.031.1011964
CAACGCA789050.029.8231535
AACGCAG825350.028.6115556
GTACATG645350.025.3302151
ACGCAGA940000.025.0684387
CGCAGAG942650.024.930598
TACATGG638100.024.536572
GCAGAGT997050.023.2643999
ACATGGG671200.022.2647023
CATGGGG341800.020.7077064
GAGTACT601000.020.69822312-13
CAGAGTA962450.020.41479310-11
CATGGGT186050.020.1216984
GTACTTT621700.019.57060214-15
Produced by FastQC (version 0.11.5)