FastQCFastQC Report
Thu 2 Jun 2016
DRR015190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785634
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302490.21942407581689752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255270.18517102659188545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147810.10722031355249964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274300.053.678071
GTATCAA508900.040.709941
GTGGTAT107000.034.518671
ATCAACG643150.031.8992583
TGGTATC113850.031.7212922
TCAACGC655150.031.3406184
TATCAAC666150.030.9628562
CAACGCA726450.028.2358195
AACGCAG750000.027.3887336
GTACATG703750.025.4843941
ACGCAGA812350.025.267117
TACATGG691050.025.2472082
CGCAGAG819150.025.028868
CATGGGT225000.023.3151424
ACATGGG714300.023.2707443
GCAGAGT878200.023.0804339
CATGGGG306050.020.8009384
GCCGTAT23350.020.17162590-91
ATGCGGG28000.019.9875898
AATGCGG29550.019.5556477
Produced by FastQC (version 0.11.5)